Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus-induced gene in the mouse

EMBO J. 1987 Mar;6(3):651-7. doi: 10.1002/j.1460-2075.1987.tb04804.x.


A genomic clone encompassing the entire coding region of a murine gene homologous to human erythroid potentiating activity/tissue inhibitor of metalloproteinase (EPA/TIMP) was isolated and sequenced. Based on alignment with human EPA/TIMP cDNAs we deduce a structure comprising five exons and four introns extending over 4.3 kb of DNA. In mouse and hamster cell lines transcription from this gene and interferon genes is induced by Newcastle Disease virus (NDV). Examination of the 5'-flanking sequences of the gene reveals a set of repeated elements with structural similarity to those previously described as inducer-responsive elements in the human IFN-beta 1 gene. The 4.3-kb DNA fragment encompassing the homologous murine EPA/TIMP gene was transfected into human T98G cells and transfectants tested for NDV inducibility. In contrast to the endogenous human gene, the integrated murine EPA/TIMP gene was NDV-inducible and TIMP activity was detectable in the cell culture fluid.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Transformation, Viral*
  • DNA / isolation & purification
  • DNA Restriction Enzymes
  • Endopeptidases / genetics*
  • Enzyme Inhibitors / genetics*
  • Exons
  • Genes*
  • Genes, Viral*
  • Humans
  • Metalloendopeptidases
  • Metalloproteins / genetics*
  • Mice
  • Newcastle disease virus / genetics*
  • Sequence Homology, Nucleic Acid
  • Tissue Inhibitor of Metalloproteinases


  • Enzyme Inhibitors
  • Metalloproteins
  • Tissue Inhibitor of Metalloproteinases
  • DNA
  • DNA Restriction Enzymes
  • Endopeptidases
  • Metalloendopeptidases