Abstract
In fission yeast the ability to undergo meiosis and sporulation is conferred by the matP+ and matM+ genes of the mating-type locus. Inactivation of ran1+, a negative regulator of meiosis, is thought to be an essential step in meiotic initiation. We have isolated a further meiotic control gene mei3+, and have shown the following: a null allele of mei3 totally inhibits meiosis; the mei3+ RNA transcript and its translational product are expressed only in matP+/matM+ diploids entering meiosis; forced expression of mei3+ in vegetative cells provokes haploid meiosis and sporulation. We suggest that the product of mei3+ gene, a protein of 21 kd, initiates meiosis by inactivating ran1+.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Amino Acid Sequence
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Base Sequence
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DNA Restriction Enzymes
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Escherichia coli / genetics
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Fungal Proteins / genetics*
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Genes*
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Genes, Fungal*
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Genes, Mating Type, Fungal*
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Genetic Vectors
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Mating Factor
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Meiosis
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Molecular Sequence Data
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Peptides / genetics*
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Protein Biosynthesis
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Saccharomycetales / genetics*
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Schizosaccharomyces / cytology
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / physiology
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Schizosaccharomyces pombe Proteins*
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Spores, Fungal / physiology
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Transcription, Genetic
Substances
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Fungal Proteins
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MEI3 protein, S pombe
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Peptides
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Schizosaccharomyces pombe Proteins
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Mating Factor
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DNA Restriction Enzymes