The product of the mei3+ gene, expressed under control of the mating-type locus, induces meiosis and sporulation in fission yeast

EMBO J. 1987 Mar;6(3):729-36.

Abstract

In fission yeast the ability to undergo meiosis and sporulation is conferred by the matP+ and matM+ genes of the mating-type locus. Inactivation of ran1+, a negative regulator of meiosis, is thought to be an essential step in meiotic initiation. We have isolated a further meiotic control gene mei3+, and have shown the following: a null allele of mei3 totally inhibits meiosis; the mei3+ RNA transcript and its translational product are expressed only in matP+/matM+ diploids entering meiosis; forced expression of mei3+ in vegetative cells provokes haploid meiosis and sporulation. We suggest that the product of mei3+ gene, a protein of 21 kd, initiates meiosis by inactivating ran1+.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • DNA Restriction Enzymes
  • Escherichia coli / genetics
  • Fungal Proteins / genetics*
  • Genes*
  • Genes, Fungal*
  • Genes, Mating Type, Fungal*
  • Genetic Vectors
  • Mating Factor
  • Meiosis
  • Molecular Sequence Data
  • Peptides / genetics*
  • Protein Biosynthesis
  • Saccharomycetales / genetics*
  • Schizosaccharomyces / cytology
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / physiology
  • Schizosaccharomyces pombe Proteins*
  • Spores, Fungal / physiology
  • Transcription, Genetic

Substances

  • Fungal Proteins
  • MEI3 protein, S pombe
  • Peptides
  • Schizosaccharomyces pombe Proteins
  • Mating Factor
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X05142