Growth and hepatospecific gene expression of human hepatoma cells in a defined medium

In Vitro Cell Dev Biol. 1987 May;23(5):349-54. doi: 10.1007/BF02620991.


The production of albumin, alpha-fetoprotein (AFP), and alpha-1 antitrypsin has been compared among human hepatoma cells cultured in medium containing serum, medium containing hormones and growth factors, and a basal medium containing selenium as the only supplement. Growth is sustained in all three media, and the expression of all three proteins was maintained for over 4 mo. in the various media. However, the quantitative production of albumin and AFP were dramatically different in the three media. Two hormones, insulin and triiodothyronine, influenced the level of secreted proteins. Triiodothyronine increases the amount of secreted albumin whereas insulin at 10 micrograms/ml reduced the level of total secreted protein.

Publication types

  • Comparative Study

MeSH terms

  • Albumins / metabolism
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / physiopathology*
  • Cell Division
  • Cell Line
  • Humans
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / physiopathology*
  • Neoplasm Proteins / metabolism*
  • Selenium / metabolism
  • Time Factors


  • Albumins
  • Neoplasm Proteins
  • Selenium