The human pyruvate dehydrogenase complex. Isolation of cDNA clones for the E1 alpha subunit, sequence analysis, and characterization of the mRNA

J Biol Chem. 1987 May 25;262(15):7398-403.

Abstract

cDNA clones corresponding to the entire length of mRNA for the alpha subunit of human pyruvate dehydrogenase (EC 1.2.4.1), the E1 component of the pyruvate dehydrogenase complex, have been isolated from liver cDNA libraries. Two classes of cDNA clones were obtained and these correspond to two forms of pyruvate dehydrogenase E1 alpha mRNA. Both mRNA species have been demonstrated in a variety of human tissues and cultured fibroblasts. The cDNA sequence has been determined and, from it, the protein sequence of the human E1 alpha subunit was deduced. The protein is synthesized with a typical mitochondrial import leader sequence and the peptide bond at which this sequence is cleaved after transport into the mitochondrion has been determined by direct amino acid sequencing of the mature E1 alpha subunit. The human pyruvate dehydrogenase E1 alpha subunit contains identical phosphorylation sites to those found in the corresponding porcine protein. Preliminary studies of pyruvate dehydrogenase E1 alpha mRNA in cultured fibroblasts from patients with severe pyruvate dehydrogenase deficiency have revealed considerable heterogeneity as would be expected from protein studies.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA / genetics*
  • DNA / isolation & purification
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • Fibroblasts / enzymology
  • Humans
  • Liver / analysis
  • Nucleic Acid Hybridization
  • Phosphorylation
  • Pyruvate Dehydrogenase Complex / genetics*
  • Pyruvate Dehydrogenase Complex Deficiency Disease
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics*
  • Tissue Distribution

Substances

  • DNA, Recombinant
  • Pyruvate Dehydrogenase Complex
  • RNA, Messenger
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J02734