Downregulation of miR-638 promotes progression of breast cancer and is associated with prognosis of breast cancer patients

Onco Targets Ther. 2018 Oct 12:11:6871-6877. doi: 10.2147/OTT.S182034. eCollection 2018.


Background: Breast cancer is the most common tumor among women. miR-638 has been demonstrated to play an important role in various cancers.

Purpose: In this study, we aimed to investigate the function and prognostic value of miR-638 in breast cancer.

Methods: Quantitative real-time polymerase chain reaction analysis was used to evaluate the expression of miR-638 in breast cancer tissues and cell lines. The correlation of miR-638 with clinicopathological features was analyzed using the chi-squared test. Kaplan-Meier survival analysis and Cox regression assay were performed to investigate the prognostic value of miR-638 in breast cancer patients. The effects of miR-638 on the biological behavior of breast cancer cells were evaluated using functional assays.

Results: The expression of miR-638 was downregulated in breast cancer tissues and cell lines (all P<0.05). Decreased expression of miR-638 was significantly correlated with lymph node metastasis (P=0.015) and TNM stage (P=0.021). Patients with low miR-638 expression had shorter overall survival compared with those with high levels (Log-rank P=0.025). The miR-638 could be considered as an independent prognostic factor for the patients (HR =0.321, 95% CI =0.117-0.882, P=0.027). Downregulation of miR-638 was capable of promoting cell proliferation, migration, and invasion in MDA-MB-231 and MCF-7 cells.

Conclusion: All the results indicate that miR-638 is a tumor suppressor in breast cancer and is involved in the progression of breast cancer. Thus, it may serve as a prognostic biomarker for breast cancer.

Keywords: breast cancer; cell proliferation; invasion; miR-638; migration; prognosis.