Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus

J Med Virol. 1987 May;22(1):45-56. doi: 10.1002/jmv.1890220107.


Most hepatitis A virus (HAV) replication in cell culture has been reported to be nonlytic and relatively slow. A rapidly replicating isolate of strain HM-175 from persistently infected, serially passed cell cultures (pHM-175) was found to induce a cytopathic effect. This observation allowed the development of a classic plaque assay for pHM-175 in FRhK-4 cells. The plaques were neutralized by polyclonal and monoclonal antisera to HAV.

MeSH terms

  • Animals
  • Cell Line
  • Cell Survival
  • Cytopathogenic Effect, Viral
  • Hepatitis A Antibodies
  • Hepatitis Antibodies / immunology
  • Hepatovirus / growth & development*
  • Hepatovirus / immunology
  • Hepatovirus / physiology
  • Neutralization Tests
  • Viral Plaque Assay*


  • Hepatitis A Antibodies
  • Hepatitis Antibodies