Lipid profiling of C. elegans strains administered pro-longevity drugs and drug combinations

Sci Data. 2018 Oct 23;5:180231. doi: 10.1038/sdata.2018.231.

Abstract

We report the effect of four lifespan modifying drugs and of synergistic combinations of these drugs on lipid profile in Caenorhabditis elegans. We employ ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) to compare the abundance of lipid species in treated and control animals. Adult nematodes were treated with rapamycin, rifampicin, psora-4 and allantoin and combinations of these compounds and the resulting change in lipid profiles, specifically in those of triacylglycerol (TAG), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were determined. We quantified changes resulting from treatment with the drug combinations relative to untreated controls and relative to animals treated with each constituent single drugs. We further determined the dependence of changes in lipid profiles on genes known to affect lipid metabolism using strains carrying mutations in these pathways. In particular, we determined lipid profiles in a genetic model of caloric restriction (eat-2), a strain lacking homolog of TGFβ (daf-7) and in a strain lacking the SREBP/sbp-1 transcription factor.

Publication types

  • Dataset
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism
  • Caloric Restriction
  • Drug Combinations
  • Lipid Metabolism*
  • Longevity / drug effects*
  • Phosphatidylcholines / metabolism
  • Phosphatidylethanolamines / metabolism
  • Receptors, Nicotinic / genetics
  • Receptors, Nicotinic / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / metabolism
  • Triglycerides / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • DAF-7 protein, C elegans
  • Drug Combinations
  • Eat-2 protein, C elegans
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Receptors, Nicotinic
  • SBP-1 protein, C elegans
  • Transcription Factors
  • Transforming Growth Factor beta
  • Triglycerides
  • phosphatidylethanolamine

Associated data

  • figshare/10.6084/m9.figshare.6739232.v3
  • figshare/10.6084/m9.figshare.6739241
  • figshare/10.6084/m9.figshare.6739247
  • figshare/10.6084/m9.figshare.6739250