Characterization of Schinus lentiscifolius Marchand (Anacardiaceae) Bark Extract and Its Effects on Lymphocyte Oxidative Stress and Heat Shock Response

Chem Biodivers. 2019 Jan;16(1):e1800303. doi: 10.1002/cbdv.201800303. Epub 2018 Dec 13.


Schinus lentiscifolius Marchand has been used in folk medicine to treat immunoinflammatory related diseases, which are marked by OS and altered HSR. Our study aimed to evaluate OS and HSR in lymphocytes treated with S. lentiscifolius bark extracts. S. lentiscifolius barks were partitioned with solvents to obtain hexane (SL-HEX), ethyl acetate (SL-ACOET) and methanol (SL-MEOH) extracts, and the presence of bioactive compounds was evaluated by thin layer chromatography. Total phenols were measured by the Folin-Ciocalteu method and flavonoids were identified by HPLC-DAD-ESI-MS/MS. Antioxidant capacity was verified by DPPH method, cell viability by Trypan Blue method, lipid peroxidation by TBARS and HSP70 by immunoblotting. The SL-ACOET extract presented higher content of phenolic compounds and antioxidant activity in vitro. It was able to reduce lipid peroxidation levels in lymphocytes induced by H2 O2 and improved cell viability. The SL-ACOET extract inhibited HSR by a decrease in both intracellular content and release of 70 kDa heat shock proteins (HSP70) and also by decrease extra-to-intracellular HSP70 ratio in lymphocytes submitted to heat shock (2 h, 41 °C). S. lentiscifolius bark extract has antioxidant activity and inhibitory effect on HSR probably due to the presence of polyphenols as the flavonoids quercetin and kaempferol.

Keywords: HSP70; Schinus lentiscifolius; antioxidant activity; biological activity; flavonoids; lipid peroxidation; oxidative stress.

MeSH terms

  • Anacardiaceae / chemistry*
  • Animals
  • Antioxidants / pharmacology*
  • Cell Survival / drug effects
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Thin Layer
  • Female
  • Flavonoids / analysis
  • HSP70 Heat-Shock Proteins / metabolism
  • Heat-Shock Response / drug effects*
  • Hydrogen Peroxide / metabolism
  • Kaempferols / pharmacology
  • Lipid Peroxidation / drug effects
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism*
  • Oxidative Stress / drug effects*
  • Phenols / analysis
  • Plant Bark / chemistry*
  • Plant Extracts / pharmacology*
  • Quercetin / pharmacology
  • Rats, Wistar
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Tandem Mass Spectrometry / methods
  • Thiobarbituric Acid Reactive Substances / metabolism


  • Antioxidants
  • Flavonoids
  • HSP70 Heat-Shock Proteins
  • Kaempferols
  • Phenols
  • Plant Extracts
  • Thiobarbituric Acid Reactive Substances
  • kaempferol
  • Quercetin
  • Hydrogen Peroxide