A new hepatitis E virus genotype 2 strain identified from an outbreak in Nigeria, 2017

Virol J. 2018 Oct 23;15(1):163. doi: 10.1186/s12985-018-1082-8.


Background: In 2017 the Nigerian Ministry of Health notified the World Health Organization (WHO) of an outbreak of hepatitis E located in the north-east region of the country with 146 cases with 2 deaths. The analysis of the hepatitis E virus (HEV) genotypes responsible for the outbreak revealed the predominance of HEV genotypes 1 (HEV-1) and 2 (HEV-2). Molecular data of HEV-2 genomes are limited; therefore we characterized a HEV-2 strain of the outbreak in more detail.

Finding: The full-length genome sequence of an HEV-2 strain (NG/17-0500) from the outbreak was amplified using newly designed consensus primers. Comparison with other HEV complete genome sequences, including the only HEV-2 strain (Mex-14) with available complete genome sequences and the availability of data of partial HEV-2 sequences from Sub-Saharan Africa, suggests that NG/17-0500 belongs to HEV subtype 2b (HEV-2b).

Conclusions: We identified a novel HEV-2b strain from Sub-Saharan Africa, which is the second complete HEV-2 sequence to date, whose natural history and epidemiology merit further investigation.

Keywords: Complete genome; HEV genotype 2; HEV subtype 2b; Hepatitis E virus; Nigeria; Outbreak.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Complementary / genetics
  • Disease Outbreaks*
  • Genome, Viral / genetics*
  • Genotype
  • Hepatitis E / blood
  • Hepatitis E / epidemiology*
  • Hepatitis E / virology*
  • Hepatitis E virus / classification*
  • Hepatitis E virus / genetics
  • Hepatitis E virus / isolation & purification*
  • Humans
  • Nigeria / epidemiology
  • Phylogeny
  • RNA, Viral / blood
  • RNA, Viral / genetics*
  • Sequence Analysis, DNA
  • Sequence Analysis, Protein


  • DNA, Complementary
  • RNA, Viral