Characterization of glucose-stimulated insulin release protocols in african green monkeys (Chlorocebus aethiops)

J Med Primatol. 2019 Feb;48(1):10-21. doi: 10.1111/jmp.12374. Epub 2018 Oct 25.

Abstract

Background: Management of diabetes remains a major health and economic challenge, demanding test systems in which to develop new therapies. These studies assessed different methodologies for determining glucose tolerance in green monkeys.

Methods: Twenty-eight African green monkeys between 4 and 24 years old underwent single or repeat intravenous glucose tolerance testing (IVGTT), oral glucose tolerance testing (OGTT), and/or graded glucose infusion testing.

Results: Geriatric monkeys exhibited glucose intolerance with impaired glucose-stimulated insulin secretion following IVGTT. Repeat IVGTT and OGTT assessments were inconsistent. Monkeys with low glucose-stimulated insulin secretion after graded glucose infusion exhibited elevated blood glucose levels.

Conclusion: IVGTT and graded glucose infusion protocols revealed differences in glucose tolerance among green monkeys at single time points, including age-dependent differences suggestive of shifts in pancreatic beta-cell functional capacity, but care should be applied to study design and the interpretation of data in the setting of longitudinal studies.

Keywords: African green monkey; aging; diabetes; graded glucose infusion; insulinopenic; intravenous glucose tolerance test; nonhuman primates; oral glucose tolerance test; plasma insulin; vervet.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chlorocebus aethiops / blood
  • Chlorocebus aethiops / physiology*
  • Female
  • Glucose Tolerance Test / statistics & numerical data*
  • Insulin Secretion*
  • Male