Yeast pyruvate carboxylase: gene isolation

Biochem Biophys Res Commun. 1987 May 29;145(1):390-6. doi: 10.1016/0006-291x(87)91334-9.

Abstract

To improve our understanding of pyruvate carboxylase (PC)(EC 6.4.1.1) structure and the evolution of the biotin-dependent carboxylases we have isolated and sequenced a yeast (Saccharomyces cerevisiae) genomic DNA fragment encoding PC. The identity of the cloned gene was confirmed by comparing the encoded protein with the sequence of a 26 amino acid biotin-containing peptide isolated from yeast PC. The yeast PC sequence is homologous (43% amino acid homology) to the rat PC sequence, although the carboxyl-terminus was found to be 44 residues from the biotinyl-lysine whereas in all biotin carboxylases sequenced to date the biotin is 35 residues from the carboxyl-terminus.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Restriction Enzymes
  • DNA, Fungal / isolation & purification
  • Genes*
  • Genes, Fungal*
  • Pyruvate Carboxylase / genetics*
  • Rats
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Sequence Homology, Nucleic Acid
  • Species Specificity

Substances

  • DNA, Fungal
  • DNA Restriction Enzymes
  • Pyruvate Carboxylase

Associated data

  • GENBANK/J03889
  • GENBANK/M16595