Multispectroscopic and Computational Investigation of ct-DNA Binding Properties with Hydroxybenzylidene Containing Tetrahydrocarbazole Derivative

J Fluoresc. 2019 Jan;29(1):101-110. doi: 10.1007/s10895-018-2314-4. Epub 2018 Oct 25.

Abstract

Mode of interaction of a new tetrahydrocarbazole derivative with ct-DNA has been investigated systematically using fluorescence spectroscopy, UV-Vis spectroscopy and circular dichroism spectroscopy. It is concluded that TAH could intercalate into the base pairs of ct-DNA, and the fluorescence quenching by ct-DNA was static quenching type. Beside the multispectroscopic results, computational studies were done. Molecular docking results revealed that the TAH-DNAs complexes might be classified as druggable molecule in drug design. Additionally, DNA binding studies exhibited that TAH complexes have different interaction and orientation abilities to each DNA isomer. Combination of experimental and computational data showed that reported TAH is promising structure and deserves further applications.

Keywords: Fluorescence; Groove binding; Stern-Volmer; Tetrahydrocabazoles; ct-DNA.

MeSH terms

  • Animals
  • Binding Sites
  • Carbazoles / chemistry*
  • Cattle
  • Circular Dichroism
  • DNA / chemistry*
  • Hydroxybenzoates / chemistry*
  • Molecular Docking Simulation*
  • Molecular Structure
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet

Substances

  • Carbazoles
  • Hydroxybenzoates
  • DNA
  • calf thymus DNA