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. 2018 Oct;82(4):256-263.

Molecular epidemiology, antimicrobial susceptibility, and pulsed-field gel electrophoresis genotyping of Pseudomonas aeruginosa isolates from mink

Yongda Zhao  1 , Lili Guo  1 , Jie Li  1 , Binghu Fang  1 , Xianhui Huang  1
Affiliations
Free PMC article

Molecular epidemiology, antimicrobial susceptibility, and pulsed-field gel electrophoresis genotyping of Pseudomonas aeruginosa isolates from mink

Yongda Zhao et al. Can J Vet Res. 2018 Oct.
Free PMC article

Abstract
in English, French

Pseudomonas aeruginosa is an important animal pathogen and contributes to hemorrhagic pneumonia in mink. Between April 2011 and December 2016, samples of lung, liver, and spleen were collected from mink with this disease on 11 mink farms in 5 Chinese provinces. From these samples, we obtained 98 isolates of P. aeruginosa that belonged to 5 serotypes: G (n = 58), I (n = 15), C (n = 8), M (n = 5), and B (n = 2); 10 isolates were not typeable (10/98). More than 90% of the isolates formed biofilms, and 85% produced slime. All 98 isolates were resistant to 10 antibiotics (oxacillin, ampicillin, penicillin G, amoxicillin, ceftriaxone, cefazolin, cefaclor, tilmicosin, tildipirosin, and sulfonamide). However, almost all were susceptible to gentamicin, polymyxin B, and amikacin. We identified 56 unique genotypes by pulsed-field gel electrophoresis. These findings have revealed genetic diversity and high antimicrobial resistance in P. aeruginosa isolated from mink with hemorrhagic pneumonia and will facilitate the prevention and control of this disease.

Pseudomonas aeruginosa est un agent pathogène animal important et contribue à la pneumonie hémorragique du vison. Entre avril 2011 et décembre 2016, des échantillons de poumon, foie, et rate ont été prélevés de visons avec cette condition dans 11 élevages retrouvés dans cinq provinces chinoises. À partir de ces échantillons nous avons obtenu 98 isolats de P. aeruginosa qui appartenaient à cinq sérotypes : G (n = 58), I (n = 15), C (n = 8), M (n = 5), et B (n = 2); 10 isolats étaient non-typables (10/98). Plus de 90 % des isolats produisaient du biofilm, et 85 % produisaient de la substance visqueuse. Les 98 isolats étaient résistants à 10 antibiotiques (oxacilline, ampicilline, pénicilline G, amoxicilline, ceftriaxone, céfazolin, céfaclor, tilmicosine, tildipirosine, et sulfonamide). Toutefois, presque tous étaient sensibles à la gentamycine, la polymyxine B, et l’amikacine. Nous avons identifié 56 génotypes uniques par électrophorèse en champs pulsés. Ces résultats ont révélé une diversité génétique et une résistance élevée aux antibiotiques chez les isolats de P. aeruginosa provenant de visons avec la pneumonie hémorragique et faciliteront la prévention et la maitrise de cette maladie.(Traduit par Docteur Serge Messier).

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Figures

Figure 1
Figure 1
Dendrogram of banding patterns obtained by pulsed-field gel electrophoresis of digested DNA from 98 Pseudomonas aeruginosa isolates.
Figure 2
Figure 2
Swimming, swarming, and twitching of the isolates.
See this image and copyright information in PMC

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