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. 2018 Oct;82(4):316-321.

Competitive Luminex immunoassays for detection of antibodies to foot-and-mouth disease and vesicular stomatitis viruses in multiple susceptible hosts

Charles Nfon  1 , Diana Lusansky  1 , Melissa Goolia  1 , Ming Yang  1 , Kate Hole  1 , Leanne McIntyre  1
Affiliations

Competitive Luminex immunoassays for detection of antibodies to foot-and-mouth disease and vesicular stomatitis viruses in multiple susceptible hosts

Charles Nfon et al. Can J Vet Res. 2018 Oct.

Abstract
in English, French

Foot-and-mouth disease (FMD) and vesicular stomatitis (VS) cause such similar clinical signs and lesions that laboratory tests are required to distinguish between infections caused by each virus. Using mouse anti-foot-and-mouth disease virus (FMDV) 3B monoclonal or polyclonal anti-vesicular stomatitis virus-New Jersey (VSV-NJ) antibodies and recombinant FMDV 3ABC or VSV-NJ glycoprotein (G) antigens coated to MagPlex beads, competitive Luminex immunoassays (cLIAs) were developed for FMDV and VSV-NJ, respectively. The cLIAs successfully detected antibodies to FMDV 3ABC and VSV-NJ G in sera from infected animals. The diagnostic sensitivity and specificity were 93% and 98%, respectively for FMDV and 93% and 95.4%, respectively for VSV-NJ. These cLIAs are potential alternatives for competitive enzyme-linked immunosorbent assays (cELISAs) and provide the opportunity for multiplexing to reduce time and the amount of serum required for testing.

La fièvre aphteuse (FA) et la stomatite vésiculaire (SV) causent des signes cliniques et des lésions tellement similaires que des tests de laboratoire sont requis afin de distinguer entre les infections causées par chaque virus. En utilisant un anticorps monoclonal 3B de souris anti-virus de la fièvre aphteuse (VFA) ou un anticorps polyclonal anti-virus de stomatite vésiculaire-New Jersey (VSV-NJ) et des billes MagPlex enduites d’antigènes de VFA recombinant 3ABC ou de glycoprotéine G de VSV-NJ, des immuno-essais Luminex compétitifs (cLIAs) furent développés pour le VFA et le VSV-NJ, respectivement. Les cLIAs ont détecté avec succès des anticorps contre VFA 3ABC et VSV-NJ G dans le sérum d’animaux infectés. La sensibilité et spécificité diagnostiques étaient de 93 % et 98 %, respectivement pour le VFA et de 93 % et 95,4 %, respectivement pour le VSV-NJ. Ces cLIAs sont des alternatives potentielles pour les épreuves ELISA compétitives et fournissent l’opportunité de multiplexer afin de réduire le temps et la quantité de sérum requis pour les tests.(Traduit par Docteur Serge Messier).

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Figures

Figure 1
Figure 1
Box plots of the distribution of A — negative sera (n = 737) and positive sera (n = 72) tested for antibodies to foot-and-mouth virus 3B on the competitive Luminex immunoassay (cLIA); B — negative sera (n = 348) and positive sera (n = 86) tested for antibodies to vesicular stomatitis virus-New Jersey serotype glycoprotein on the cLIA.
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