Analysis of DNA surrounding the breakpoints of chromosomal translocations involving the beta T cell receptor gene in human lymphoblastic neoplasms

Cell. 1987 Jul 3;50(1):107-17. doi: 10.1016/0092-8674(87)90667-2.


DNA containing breakpoints of two different t(7;9) chromosomal translocations was cloned from the T lymphoblastic tumor cell lines SUP-T1 and SUP-T3. Sequence analysis of DNA from the t(7;9)(q34;q34.3) translocation of SUP-T1 revealed that chromosome 9 DNA had recombined with DNA 5' to rearranged D-J regions in the beta T cell receptor gene of chromosome 7. Restriction analysis and hybridization studies using DNA fragments cloned from the t(7;9)(q34;32) translocation of SUP-T3 confirmed that beta T cell receptor DNA is also joined to the DNA of chromosome 9 in these cells. Using hybridization probes for the two breakpoints, several other cases of T lymphoblastic tumors were shown to possess DNA rearrangements near the 9q34.3 and 9q32 sites. Hybridization with the 9q34.3 probe detected multiple transcripts in SUP-T1 RNA and small amounts of larger transcripts in T cells lacking the t(7;9)(q34;q34.3) translocation. This work directly demonstrates that the beta T cell receptor locus may frequently be involved in chromosomal translocations within T lymphoblastic neoplasms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • Chromosomes, Human, Pair 7*
  • Chromosomes, Human, Pair 9*
  • DNA Restriction Enzymes
  • DNA, Neoplasm / genetics
  • Humans
  • Leukemia, Lymphoid / genetics*
  • Peptide Fragments / genetics*
  • RNA, Messenger / genetics
  • RNA, Neoplasm / genetics
  • Receptors, Antigen, T-Cell / genetics*
  • Receptors, Antigen, T-Cell, alpha-beta
  • Recombination, Genetic
  • T-Lymphocytes / physiology*
  • Translocation, Genetic*


  • DNA, Neoplasm
  • Peptide Fragments
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, alpha-beta
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M16771
  • GENBANK/M16834