Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase

Cell. 1987 Jul 17;50(2):277-87. doi: 10.1016/0092-8674(87)90223-6.


We have isolated three genes (TPK1, TPK2, and TPK3) from the yeast S. cerevisiae that encode the catalytic subunits of the cAMP-dependent protein kinase. Gene disruption experiments demonstrated that no two of the three genes are essential by themselves but at least one TPK gene is required for a cell to grow normally. Comparison of the predicted amino acid sequences of the TPK genes indicates conserved and variable domains. The carboxy-terminal 320 amino acid residues have more than 75% homology to each other and more than 50% homology to the bovine catalytic subunit. The amino-terminal regions show no homology to each other and are heterogeneous in length. The TPK1 gene carried on a multicopy plasmid can suppress both a temperature-sensitive ras2 gene and adenylate cyclase gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Restriction Enzymes
  • Genes*
  • Genes, Fungal*
  • Genotype
  • Macromolecular Substances
  • Plasmids
  • Protein Kinases / genetics*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*


  • Macromolecular Substances
  • Protein Kinases
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M17072
  • GENBANK/M17073
  • GENBANK/M17074