Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites

Gene. 1987;51(2-3):275-80. doi: 10.1016/0378-1119(87)90316-7.

Abstract

The construction of a new wide-host-range, restriction-site bank, cosmid-cloning vehicle (pJRD215) is described. The wide-host-range properties and the ability to be transferred by conjugation, extend genetic engineering to those Gram-negative species that cannot be transformed. The vector permits the cloning of genes from Gram-negative bacteria using a complementation screening procedure in a mutant host. This procedure is simplified by the possibility of construction of a cosmid gene bank so that only a few hundred clones need to be screened. Subsequent subcloning of the gene of interest is facilitated by the presence of at least 23 unique cloning sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular / methods*
  • Conjugation, Genetic
  • Cosmids*
  • DNA Replication
  • DNA Restriction Enzymes / metabolism
  • Genetic Vectors*
  • Gram-Negative Bacteria
  • Gram-Positive Bacteria

Substances

  • DNA Restriction Enzymes

Associated data

  • GENBANK/M16198