Abstract
Cultured SK-OS-10 cells (human osteogenic sarcoma metastatic to lung) shed microvesicles (dia. 300-1000 nm) that contained procoagulant and proaggregatory activities inhibitable by hirudin, by anti-tissue factor antibody and by phospholipase A2. These results show that SK-OS-10 cells belong to a group including U87MG human glioblastoma and HL-60 promyelocytic leukemia in which these activities are due to a thrombin-dependent mechanism arising from the presence of tissue factor on the surface of the tumor cells and their shed microvesicles.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies
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Blood Platelets / physiology*
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Cell Line
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Creatine Kinase / pharmacology
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Hirudins / pharmacology
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Humans
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Inclusion Bodies / metabolism
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Lung Neoplasms / secondary*
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Osteosarcoma / metabolism*
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Osteosarcoma / pathology
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Phosphocreatine / pharmacology
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Phospholipases / pharmacology
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Platelet Activating Factor
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Platelet Aggregation / drug effects
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Thromboplastin / immunology
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Thromboplastin / pharmacology*
Substances
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Antibodies
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Hirudins
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Platelet Activating Factor
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Phosphocreatine
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Thromboplastin
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Creatine Kinase
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Phospholipases