Replacement of glycoprotein B gene sequences in herpes simplex virus type 1 strain ANG by corresponding sequences of the strain KOS causes changes of plaque morphology and neuropathogenicity

J Gen Virol. 1987 Jul;68 ( Pt 7):1909-19. doi: 10.1099/0022-1317-68-7-1909.

Abstract

DNA sequences encoding glycoprotein B (gB) derived from herpes simplex virus type 1 (HSV-1) strain KOS321 were transferred to HSV-1 ANG. In cotransfection experiments the cloned HSV-1 KOS BamHI G fragment served as donor, and genomic DNA of two ANG variants as recipients. One of these variants, HSV-1 ANG path, expresses gC and the other, C18, was a spontaneous gC-negative mutant. Both ANG strains are of the syncytial (syn) phenotype whereas HSV-1 KOS321 is non-syncytial (syn+). Recombinants were identified by means of a monoclonal antibody which selectively recognizes gBKOS. Among the HSV-1 ANG path/gBKOS recombinants, the majority displayed an altered plaque morphology, i.e. they were of the syn+ phenotype. In contrast all of the C18/gBKOS recombinants were of the syn phenotype. The possibility that the mutant C18 carries a syn mutation not present in the parental strain could be excluded. Marker transfer experiments involving subfragments of the gB gene mapped the syn mutation of HSV-1 ANG path to a locus within the gene that has been previously termed syn 3. Subclones of HSV-1 ANG path were established either directly or after intermittent transfection or cotransfection with the KOS BamHI G fragment. The pathogenicity in mice of these clones was compared. The data obtained indicated that at high frequency, the BamHI G fragment confers apathogenicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Fusion
  • Cytopathogenic Effect, Viral
  • DNA, Recombinant
  • DNA, Viral / genetics*
  • Encephalitis / etiology
  • Encephalitis / physiopathology
  • Genes
  • Genes, Viral
  • Herpes Simplex / etiology
  • Herpes Simplex / physiopathology
  • Mice
  • Mice, Inbred DBA
  • Simplexvirus / genetics*
  • Simplexvirus / pathogenicity
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / physiology

Substances

  • DNA, Recombinant
  • DNA, Viral
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus
  • glycoprotein gC, herpes simplex virus type 1