Sendai virus is a member of the family Paramyxoviridae, and an enveloped virus with a negative-stranded RNA genome. Sendai virus is not pathogenic to humans, but for mice and can cause pneumonia in mice. Easy and efficient techniques for propagating Sendai virus are required for studying virus replication, virus-induced innate- and adaptive-immunity, Sendai-virus-based virotherapy and IgA nephropathy. Here, we describe a protocol for Sendai virus propagation using chicken eggs. This traditional protocol enables us to generate a large amount of virus enough for animal experiments as well as cell culture experiments in a relatively inexpensive way.
Keywords: Allantoic fluid; Chicken eggs; Harvest; Mouse parainfluenza virus; Propagation; Sendai virus.
Conflict of interest statement
Competing interests Authors declare no conflicts of interest or competing interests.
High Level Expression of Soluble Glycoproteins in the Allantoic Fluid of Embryonated Chicken Eggs Using a Sendai Virus Minigenome SystemT Corral et al. BMC Biotechnol 7, 17. PMID 17411439.The simplicity and economy of the Sendai minigenome system, together with the high yield achieved in the allantoic fluid of eggs, makes it an attractive method to express …
Quantification of Infectious Sendai Virus Using Plaque AssayN Tatsumoto et al. Bio Protoc 8 (21). PMID 30547053.Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunol …
[Effect of Urea and Uric Acid on the Hemolytic Activity of Sendai Virus]E Trybała et al. Med Dosw Mikrobiol 41 (1), 43-6. PMID 2548047.It was found that haemolytic activity of Fushimi strain of Sendai virus multiplied in allantoic cavity of chicken embryos is independent on its haemagglutinating titer an …
Sendai Virus, the Mouse Parainfluenza Type 1: A Longstanding Pathogen That Remains Up-To-DateP Faísca et al. Res Vet Sci 82 (1), 115-25. PMID 16759680. - ReviewBiologically speaking, Sendai virus (SeV), the murine parainfluenza virus type 1, is perceived as a common respiratory pathogen that is endemic in many rodent colonies th …
Sendai Virus Vectors as an Emerging Negative-Strand RNA Viral Vector SystemM Bitzer et al. J Gene Med 5 (7), 543-53. PMID 12825193. - ReviewThe power to manipulate the genome of negative-strand RNA viruses, including the insertion of additional non-viral genes, has led to the development of a new class of vir …
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