Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae

Mol Cell Biol. 1987 Apr;7(4):1371-7. doi: 10.1128/mcb.7.4.1371-1377.1987.


We have cloned a gene (BCY1) from the yeast Saccharomyces cerevisiae that encodes a regulatory subunit of the cyclic AMP-dependent protein kinase. The encoded protein has a structural organization similar to that of the RI and RII regulatory subunits of the mammalian cyclic AMP-dependent protein kinase. Strains of S. cerevisiae with disrupted BCY1 genes do not display a cyclic AMP-dependent protein kinase in vitro, fail to grow on many carbon sources, and are exquisitely sensitive to heat shock and starvation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / genetics*
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • Genes*
  • Genes, Fungal*
  • Genetic Complementation Test
  • Genetic Linkage
  • Genotype
  • Intracellular Signaling Peptides and Proteins*
  • Macromolecular Substances
  • Protein Kinases / genetics*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*


  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Macromolecular Substances
  • protein kinase modulator
  • Protein Kinases
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M15756