Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family

Nature. 1987 Jul 16-22;328(6127):221-7. doi: 10.1038/328221a0.

Abstract

Amino-acid sequences derived from complementary DNAs encoding the alpha- and beta-subunits of the GABA/benzodiazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the in vitro-generated alpha-subunit and beta-subunit RNAs in Xenopus oocytes produces a functional receptor and ion channel with the pharmacological properties characteristic of the GABAA receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression Regulation
  • Membrane Proteins / genetics
  • Multigene Family
  • Oocytes / physiology
  • Protein Conformation
  • Receptors, GABA-A / genetics*
  • Receptors, GABA-A / physiology
  • Receptors, Nicotinic / genetics
  • gamma-Aminobutyric Acid / physiology*

Substances

  • Membrane Proteins
  • Receptors, GABA-A
  • Receptors, Nicotinic
  • gamma-Aminobutyric Acid
  • DNA

Associated data

  • GENBANK/X05717
  • GENBANK/X05718