Thiol/Disulfide homeostasis in patients with rheumatoid arthritis

Rom J Intern Med. 2019 Mar 1;57(1):30-36. doi: 10.2478/rjim-2018-0025.

Abstract

Background: Oxidative stress may play an important role in rheumatoid arthritis (RA) etiopathogenesis. The thiol group is a very strong antioxidant. In this study, we aimed to investigate the presence of oxidative stress in patients with RA by evaluating thiol/disulfide homeostasis.

Material and methods: A total of 50 female RA patients and 50 healthy female controls were included in this study. Thiol and disulfide values were calculated utilizing novel methods.

Results: Native thiol (p < 0.001) and total thiol (p < 0.001) levels of RA patients were significantly lower compared to values in the control group. However, the disulfide (p < 0.001) levels of RA patients were strongly higher than in healthy individuals. A negative correlation was found between thiol and disease activity score-28 among the patients, whereas a positive correlation was found between disulfide and disease activity score-28 among the patients.

Conclusion: We found that the thiol-disulfide rate deteriorated in RA patients, with the proportion of disulfide increasing. There is a strong correlation between the decrease in thiol levels, increase in disulfide levels and the disease activity scores.

Keywords: Rheumatoid arthritis; disulfide; oxidative stress; thiol; thiol/disulfide homeostasis.

MeSH terms

  • Adult
  • Antirheumatic Agents / therapeutic use
  • Arthritis, Rheumatoid / blood*
  • Arthritis, Rheumatoid / drug therapy
  • Arthritis, Rheumatoid / physiopathology*
  • Biomarkers / blood
  • Disulfides / blood*
  • Female
  • Homeostasis*
  • Humans
  • Middle Aged
  • Oxidative Stress*
  • Sulfhydryl Compounds / blood*

Substances

  • Antirheumatic Agents
  • Biomarkers
  • Disulfides
  • Sulfhydryl Compounds