FLIRT: fast local infrared thermogenetics for subcellular control of protein function

Nat Methods. 2018 Nov;15(11):921-923. doi: 10.1038/s41592-018-0168-y. Epub 2018 Oct 30.


FLIRT (fast local infrared thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature-sensitive mutant proteins. We demonstrate that FLIRT can control temperature-sensitive proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in Caenorhabditis elegans with cell-specific and even subcellular precision.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans / radiation effects
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Differentiation
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / physiology
  • Gene Expression Regulation
  • Genetic Techniques / instrumentation*
  • Germ Cells
  • Infrared Rays*
  • Microscopy
  • Molecular Imaging / methods*
  • Mutation*
  • Receptors, Notch
  • Signal Transduction
  • Temperature*


  • Caenorhabditis elegans Proteins
  • Receptors, Notch