The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

Nat Methods. 2018 Nov;15(11):901-904. doi: 10.1038/s41592-018-0180-2. Epub 2018 Oct 30.


We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallography, X-Ray*
  • Equipment Design
  • Hydrolases / chemistry*
  • Models, Molecular
  • Protein Conformation*
  • Rhodopseudomonas / enzymology*
  • Synchrotrons / instrumentation*
  • Time Factors


  • Hydrolases
  • haloacetate dehalogenase