Orthotopic Pancreatic Tumor Mouse Models of Liver Metastasis

Methods Mol Biol. 2019;1882:309-320. doi: 10.1007/978-1-4939-8879-2_27.


The survival from pancreatic cancer is poor because most patients are diagnosed after the cancer has metastasized. Liver is the most common site of pancreatic cancer metastasis. Orthotopic mouse models of liver metastasis by intrasplenically injecting the pancreatic tumor cells are useful in studying the molecular mechanisms of metastasis and evaluating therapeutic regimens.

Keywords: Intrasplenic injection; Liver; Metastasis; Mouse model; Orthotopic; Pancreatic cancer; Splenectomy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods
  • Cell Line, Tumor
  • Culture Media, Conditioned
  • Humans
  • Liver / pathology*
  • Liver Neoplasms / pathology
  • Liver Neoplasms / secondary*
  • Mice
  • Mice, Inbred NOD
  • Pancreatic Neoplasms / pathology*
  • Xenograft Model Antitumor Assays / instrumentation
  • Xenograft Model Antitumor Assays / methods*


  • Culture Media, Conditioned