High-Throughput Single-Cell Sequencing of both TCR-β Alleles

J Immunol. 2018 Dec 1;201(11):3465-3470. doi: 10.4049/jimmunol.1800774. Epub 2018 Oct 31.


Allelic exclusion is a vital mechanism for the generation of monospecificity to foreign Ags in B and T lymphocytes. In this study, we developed a high-throughput barcoded method to simultaneously analyze the VDJ recombination status of both mouse TCR-β alleles in hundreds of single cells using next-generation sequencing.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alleles
  • Animals
  • B-Lymphocytes / immunology*
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor*
  • Genes, T-Cell Receptor beta / genetics*
  • High-Throughput Nucleotide Sequencing
  • Mice
  • Mice, Inbred C57BL
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Single-Cell Analysis
  • T-Lymphocytes / immunology*
  • V(D)J Recombination


  • Receptors, Antigen, T-Cell, alpha-beta