MicroRNA-22 Suppresses Breast Cancer Cell Growth and Increases Paclitaxel Sensitivity by Targeting NRAS

Technol Cancer Res Treat. 2018 Jan 1;17:1533033818809997. doi: 10.1177/1533033818809997.


In recent study, microRNAs have various important functions in diverse biological processes and progression of cancer. In human breast cancer, microRNA-22 has been reported to be downregulated. However, molecular mechanism of microRNA-22 in breast cancer progression and chemosensitivity has not been well studied. In our study, these results demonstrated that microRNA-22 expression levels were significantly reduced in 40 pairs of human breast cancer tissues when compared to normal tissues. Enforced expression of microRNA-22 inhibited activity of cell proliferation and cell migration in breast cancer cells. Furthermore, microRNA-22 targeted NRAS proto-oncogene, GTPase (NRAS) in breast cancer cells. The expression levels of NRAS in human clinical specimens were higher in breast cancer tissues when compared to normal tissues. Moreover, microRNA-22 sensitized breast cancer cells to paclitaxel by regulation of NRAS. Our results then demonstrated that microRNA-22 functioned as a tumor suppressor microRNA and indicated potential application for the diagnosis and treatment of cancer in the future.

Keywords: NRAS; breast cancer; miR-22; paclitaxel; tumor suppressor.

Publication types

  • Retracted Publication

MeSH terms

  • Biological Phenomena / drug effects
  • Biological Phenomena / genetics
  • Breast / drug effects
  • Breast / pathology
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Cell Movement / genetics
  • Cell Proliferation / drug effects
  • Cell Proliferation / genetics*
  • Disease Progression
  • Down-Regulation / drug effects
  • Down-Regulation / genetics
  • Female
  • GTP Phosphohydrolases / genetics*
  • Gene Expression Regulation, Neoplastic / drug effects
  • Gene Expression Regulation, Neoplastic / genetics
  • Genes, Tumor Suppressor / drug effects
  • Genes, Tumor Suppressor / physiology
  • HEK293 Cells
  • Humans
  • MCF-7 Cells
  • Membrane Proteins / genetics*
  • MicroRNAs / genetics*
  • Paclitaxel / pharmacology*
  • Proto-Oncogene Mas


  • MAS1 protein, human
  • MIRN22 microRNA, human
  • Membrane Proteins
  • MicroRNAs
  • Proto-Oncogene Mas
  • GTP Phosphohydrolases
  • NRAS protein, human
  • Paclitaxel