Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)

Biol Res. 2018 Nov 1;51(1):42. doi: 10.1186/s40659-018-0193-4.

Abstract

Background: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement.

Results: Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively.

Conclusions: From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

Keywords: Chilean berry; Gaultheria pumila; Microsatellite; Pyrosequencing.

MeSH terms

  • Alleles
  • DNA, Plant / genetics*
  • Gaultheria / genetics*
  • Genetic Variation
  • Microsatellite Repeats / genetics*
  • Polymorphism, Genetic

Substances

  • DNA, Plant