Volume microscopy in biology: FIB-SEM tomography

Caroline Kizilyaprak; York-Dieter Stierhof; Bruno M Humbel

doi:10.1016/j.tice.2018.09.006

Volume microscopy in biology: FIB-SEM tomography

Tissue Cell. 2019 Apr:57:123-128. doi: 10.1016/j.tice.2018.09.006. Epub 2018 Sep 26.

Authors

Caroline Kizilyaprak¹, York-Dieter Stierhof², Bruno M Humbel³

Affiliations

¹ Electron Microscopy Facility, University of Lausanne, Lausanne, Switzerland.
² Center for Plant Molecular Biology (ZMBP), Light and Electron Microscopy, University of Tübingen, Tübingen, Germany.
³ Electron Microscopy Facility, University of Lausanne, Lausanne, Switzerland; Imaging, Okinawa Institute of Science and Technology, Onna-son, Okinawa, Japan. Electronic address: Bruno.Humbel@oist.jp.

PMID: 30385054
DOI: 10.1016/j.tice.2018.09.006

Abstract

Volume microscopy has become an important method in cellular biology. In contrast to tedious serial sectioning volumes can now far more conveniently be obtained with serial-block face and focussed ion beam scanning electron microscopy. Serial-block face scanning electron microscopy is the instrument of choice for large volumes whereas focussed ion beam scanning electron microscopy has its merits in high voxel resolution. These aspects are discussed along with some specific applications of a focussed ion beam scanning electron microscope.

Keywords: Electron microscopy sample preparation; Focussed ion beam scanning microscopy; Serial block-face scanning microscopy; Serial sectioning; Volume microscopy.

Publication types

Review

MeSH terms

Animals
Humans
Image Processing, Computer-Assisted / methods
Imaging, Three-Dimensional / methods*
Microscopy, Electron, Scanning / methods*