Human papillomavirus type 16 DNA cooperates with activated ras in transforming primary cells

EMBO J. 1987 Jun;6(6):1741-6.

Abstract

The close association of human papillomavirus type 16 DNA with a majority of cervical carcinomas implies some role for the virus in this type of cancer. To define the transforming properties of HPV-16 DNA in vitro we have now performed transfection experiments on baby rat kidney cells using HPV-16 DNA in conjunction with an activated ras gene. We have demonstrated that a 6.6-kb DNA fragment, containing the early genes of HPV-16 under the control of Moloney murine leukaemia virus long terminal repeats (MoMuLV-LTRs), cooperates with EJ-ras in transforming these cells. Both DNAs are required and neither alone is effective. The cooperating activity appears to reside in a protein or proteins derived from the E6/E7 region of the HPV-16 genome.

MeSH terms

  • Animals
  • Cell Line
  • Cell Transformation, Viral*
  • Chromosome Deletion
  • DNA, Viral / genetics*
  • Genes, Viral*
  • Mutation
  • Nucleic Acid Hybridization
  • Oncogenes*
  • Papillomaviridae / genetics*
  • Plasmids
  • Transfection

Substances

  • DNA, Viral