Cloning of the riboB locus of Aspergillus nidulans

Gene. 1987;53(2-3):293-8. doi: 10.1016/0378-1119(87)90019-9.


We have complemented the riboB2 mutation of Aspergillus nidulans by transformation with a plasmid library of wild-type (wt) sequences. We have isolated, by marker rescue from a riboB+ transformant, a plasmid that complements riboB2 efficiently. From this plasmid we have subcloned an A. nidulans sequence that complements riboB2 efficiently and that integrates by homologous recombination at a site closely linked to the riboB locus. We conclude that this sequence contains the wt riboB+ allele.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aspergillus nidulans / genetics*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Genes
  • Genes, Fungal
  • Genetic Complementation Test
  • Mutation
  • Recombination, Genetic
  • Riboflavin / metabolism*


  • DNA Restriction Enzymes
  • Riboflavin