Laurus nobilis leaf extract controls inflammation by suppressing NLRP3 inflammasome activation

J Cell Physiol. 2019 May;234(5):6854-6864. doi: 10.1002/jcp.27434. Epub 2018 Nov 1.


Laurus nobilis Linn. (Lauraceae), commonly known as Bay, has been used as a traditional medicine in the Mediterranean and Europe to treat diverse immunological disorders. Although the effects of L. nobilis on immunosuppression have been reported, the detailed underlying mechanism remains unclear. In this study, to elucidate the anti-inflammatory mechanism of L. nobilis, we examined the effect of L. nobilis leaf extract on inflammasome activation in mouse bone marrow-derived macrophages. L. nobilis leaf extract inhibited NOD-like receptor pyrin domain-containing 3 (NLRP3) inflammasome activation, which was associated with caspase-1 activation, interleukin-1β secretion, and apoptosis-associated speck-like protein containing a CARD (ASC) pyroptosome complex formation. We also observed that 1,8-cineole, the major component of L. nobilis extract, consistently suppressed NLRP3 inflammasome activation. Furthermore, L. nobilis leaf extract attenuated the in vivo expression of proinflammatory cytokines in an acute lung injury mouse model. Our results provide the first evidence that L. nobilis leaf extract modulates inflammatory signaling by suppressing inflammasome activation.

Keywords: 1,8-cineole; Laurus nobilis L.; NLRP3; inflammasome; inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / drug therapy
  • Acute Lung Injury / metabolism
  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Apoptosis / drug effects
  • Caspase 1 / metabolism
  • Cell Line
  • Cytokines / metabolism
  • HEK293 Cells
  • Humans
  • Inflammasomes / drug effects*
  • Inflammasomes / metabolism
  • Inflammation / drug therapy*
  • Inflammation / metabolism
  • Interleukin-1beta / metabolism
  • Lauraceae / chemistry*
  • Laurus / chemistry*
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Mice, Inbred C57BL
  • NLR Family, Pyrin Domain-Containing 3 Protein / metabolism*
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Signal Transduction / drug effects


  • Anti-Inflammatory Agents
  • Cytokines
  • Inflammasomes
  • Interleukin-1beta
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Nlrp3 protein, mouse
  • Plant Extracts
  • Caspase 1