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. 2018 Dec;120(12):1349-1358.
doi: 10.1017/S0007114518002908. Epub 2018 Nov 2.

High Dosage of Zinc Modulates T-cells in a Time-Dependent Manner Within Porcine Gut-Associated Lymphatic Tissue

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High Dosage of Zinc Modulates T-cells in a Time-Dependent Manner Within Porcine Gut-Associated Lymphatic Tissue

Susanne Kreuzer-Redmer et al. Br J Nutr. .

Abstract

Zn serves as a powerful feed additive to reduce post-weaning diarrhoea in pigs. However, the mechanisms responsible for Zn-associated effects on the adaptive immune responses following feeding of a very high dosage of Zn remain elusive. In this study, we examined the T-cell response in gut-associated lymphatic tissues of seventy-two weaned piglets. Piglets received diets with 57 mg Zn/kg (low Zn concentration, LZn), 164 mg Zn/kg (medium Zn concentration, MZn) or 2425 mg Zn/kg (high Zn concentration, HZn) mg Zn/kg feed for 1, 2 or 4 weeks. We observed that feeding the HZn diet for 1 week increased the level of activated T-helper cells (CD4+ and CD8α dim) compared with feeding MZn and LZn (P<0·05). In addition, we observed higher transcript amounts of interferon γ and T-box 21 (TBET) in the HZn group compared with the MZn and LZn groups (P<0·05). A gene set enrichment analysis revealed an over-representation of genes associated with 'cytokine signalling in immune system'. Remarkably, feeding of a very high Zn dosage led to a switch in the immune response after 2 weeks. We detected higher relative cell counts of CD4+CD25high regulatory T-helper cells (P<0·05) and a higher expression of forkhead box P3 (FOXP3) transcripts (P<0·05). After 4 weeks of feeding a high-dosage Zn diet, the relative CD4+ T-cell count (P<0·05) and the relative CD8β + T-cell count (P<0·1) were reduced compared with the MZn group. We hypothesise that after 1 week the cellular T-helper 1 response is switched on and after 2 weeks it is switched off, leading to decreased numbers of T-cells.

Keywords: GATA3 GATA binding protein 3; IFNγ interferon γ; TBET T-box 21; ZIP4 solute carrier family 39 member 4 (SLC39A4); ZnT solute carrier family 30 (SLC30); BW body weight; FOXP3 forkhead box P3; GALT gut-associated lymphatic tissue; GSEA gene set enrichment analysis; HZn high Zn concentration; LZn low Zn concentration; MLN mesenteric lymph node; MZn medium Zn concentration; Nutritional immune response; Pigs; T-cells; Zinc.

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