CRISPR-Based Technologies: Impact of RNA-Targeting Systems

Mol Cell. 2018 Nov 1;72(3):404-412. doi: 10.1016/j.molcel.2018.09.018.


DNA-targeting CRISPR-Cas systems, such as those employing the RNA-guided Cas9 or Cas12 endonucleases, have revolutionized our ability to predictably edit genomes and control gene expression. Here, I summarize information on RNA-targeting CRISPR-Cas systems and describe recent advances in converting them into powerful and programmable RNA-binding and cleavage tools with a wide range of novel and important biotechnological and biomedical applications.

Keywords: CRISPR; Cas10; Cas13; Cas9; RNA editing; RNA targeting; Type III CRISPR-Cas systems; Type VI CRISPR-Cas systems; gene editing.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • CRISPR-Cas Systems / genetics*
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics
  • DNA
  • Gene Editing / methods*
  • Genetic Engineering / methods*
  • Humans
  • RNA / genetics
  • RNA, Guide / genetics


  • RNA, Guide
  • RNA
  • DNA