Short-term changes in levels of cyclic AMP, adenylate cyclase, and phosphodiesterase during the initiation of sperm motility in rainbow trout

J Exp Zool. 1987 May;242(2):199-204. doi: 10.1002/jez.1402420211.

Abstract

In order to clarify the role of the system that generates and degrades cyclic AMP during the initiation of motility of trout sperm, short-term changes in levels of intraspermatozoal cyclic AMP, adenylate cyclase, and phosphodiesterase were measured. Levels of cyclic AMP and the activity of adenylate cyclase increased and reached a maximum level 1 sec after transfer of sperm to K+-free medium, where they became motile, and then decreased rapidly. However, there were no changes in either parameter in sperm which remained immotile in K+-rich medium. In addition, an increase in the activity of phosphodiesterase was observed 4 sec later than the increase in levels of cyclic AMP and adenylate cyclase. These findings suggest that a very rapid change in the level of intracellular cyclic AMP occurs within 1 sec, at the moment of spawning, by the activation of adenylate cyclase and phosphodiesterase, and regulates the initiation of trout sperm motility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism*
  • Animals
  • Cyclic AMP / metabolism*
  • Male
  • Phosphoric Diester Hydrolases / metabolism*
  • Salmonidae / physiology*
  • Semen / analysis
  • Semen / metabolism
  • Sperm Motility*
  • Spermatozoa / analysis
  • Spermatozoa / enzymology
  • Trout / physiology*

Substances

  • Cyclic AMP
  • Phosphoric Diester Hydrolases
  • Adenylyl Cyclases