Light-Controlled Chemoenzymatic Immobilization of Proteins towards Engineering of Bioactive Papers

Chemistry. 2019 Feb 1;25(7):1746-1751. doi: 10.1002/chem.201804889. Epub 2019 Jan 2.


Efficient and reliable methods for the generation of bioactive papers are of growing interest in relation to point-of-care testing devices that do not require extensive analytical equipment. Herein, we report the immobilization of functional proteins on paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on a combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method has allowed unidirectional and covalent immobilization of several proteins displaying different functional properties, with ramifications for application in paper-based diagnostics.

Keywords: bioactive papers; biosensors; chemoenzymatic ligation; enzyme catalysis; protein immobilization; sortases.

MeSH terms

  • Amides / chemistry
  • Aminoacyltransferases / chemistry
  • Aminoacyltransferases / metabolism*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism*
  • Biocatalysis
  • Cycloaddition Reaction
  • Cysteine Endopeptidases / chemistry
  • Cysteine Endopeptidases / metabolism*
  • Fluorescent Dyes / chemistry
  • Immobilized Proteins / chemistry
  • Immobilized Proteins / metabolism
  • Light*
  • Microscopy, Confocal
  • Paper*
  • Peptides / analysis
  • Peptides / chemistry
  • Point-of-Care Systems
  • Stereoisomerism


  • Amides
  • Bacterial Proteins
  • Fluorescent Dyes
  • Immobilized Proteins
  • Peptides
  • Aminoacyltransferases
  • sortase A
  • Cysteine Endopeptidases

Grant support