CUB Domain-containing Protein 1 (CDCP1) Is Down-regulated by Active Hexose-correlated Compound in Human Pancreatic Cancer Cells

Anticancer Res. 2018 Nov;38(11):6107-6111. doi: 10.21873/anticanres.12961.

Abstract

Background/aim: We have previously reported that treatment of pancreatic cancer cells with active hexose-correlated compound (AHCC), an extract of a basidiomycete mushroom, decreases the levels of tumor-associated proteins including heat-shock protein 27 (HSP27), heat shock factor 1 (HSF1) and sex-determining region Y-box 2 (SOX2). The transmembrane glycoprotein, CUB domain-containing protein 1 (CDCP1) has been reported to be up-regulated in various cancers, and be associated with invasion and metastasis. The aim of this study was to examine the effect of AHCC on the expression of CDCP1 in KLM1-R cells.

Materials and methods: Gemcitabine-resistant pancreatic cancer cells (KLM1-R) were treated with AHCC (10 mg/ml) for 48 h. Western blot analysis of cell extracts with anti-CDCP1 or anti-actin antibodies was performed to assess the expression of CDCP1.

Results: Expression of CDCP1 was reduced by AHCC treatment of KLM1-R cells, whereas expression of actin was not affected. The ratio of intensities of CDCP1/actin in AHCC-treated KLM1-R cells was significantly suppressed (p<0.05) compared to untreated cells.

Conclusion: AHCC down-regulated CDCP1 expression and inhibited the malignant progression of pancreatic cancer cells.

Keywords: AHCC; CDCP1; metastasis; pancreatic cancer.

MeSH terms

  • Actins / biosynthesis
  • Antigens, CD / biosynthesis*
  • Antigens, Neoplasm
  • Blotting, Western
  • Cell Adhesion Molecules / biosynthesis*
  • Cell Line, Tumor
  • Deoxycytidine / analogs & derivatives
  • Deoxycytidine / pharmacology
  • Down-Regulation / drug effects
  • Drug Resistance, Neoplasm
  • Humans
  • Neoplasm Proteins / biosynthesis*
  • Pancreatic Neoplasms / drug therapy*
  • Pancreatic Neoplasms / metabolism*
  • Polysaccharides / pharmacology*

Substances

  • Actins
  • Antigens, CD
  • Antigens, Neoplasm
  • CDCP1 protein, human
  • Cell Adhesion Molecules
  • Neoplasm Proteins
  • Polysaccharides
  • Deoxycytidine
  • gemcitabine
  • Active Hexose Correlated Compound