Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure

Nat Methods. 2018 Dec;15(12):1029-1032. doi: 10.1038/s41592-018-0177-x. Epub 2018 Nov 5.


Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / ultrastructure*
  • Female
  • Fluorescent Antibody Technique
  • Imaging, Three-Dimensional / methods*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Electron / methods*
  • Microscopy, Fluorescence / methods*
  • Single-Domain Antibodies / immunology*


  • Single-Domain Antibodies