Active Components from Sea Buckthorn ( Hippophae rhamnoides L.) Regulate Hepatic Stellate Cell Activation and Liver Fibrogenesis

J Agric Food Chem. 2018 Nov 21;66(46):12257-12264. doi: 10.1021/acs.jafc.8b05306. Epub 2018 Nov 13.

Abstract

Sea buckthorn ( Hippophae rhamnoides L.) is a berry bearing multiple nutritional properties. In this study, 46 compounds were isolated from sea buckthorn berries. Preliminary data showed that the components, C13, C15, and C32, exhibited profound inhibitory effect on the activation of hepatic stellate cells (HSCs) induced by transforming growth factor-β (TGF-β) and decreased the levels of inflammatory factors. Furthermore, these compounds over-regulated the proteins of DNA damage signaling pathway and alpha-smooth muscle actin (α-SMA). Moreover, active components of sea buckthorn berry (ACSB) treatment attenuated fibrosis development in rats after bile duct ligation (BDL), reducing liver injury and inflammation, and reviving liver function in a dose-dependent manner. Moreover, ACSB down-regulated the expression of α-SMA, while over-regulating the DNA damage signaling pathway and the related genes. These suggest that ACSB inhibit DNA repair of HSCs, make them in a damaged state, inhibit the expression of TGF-β, and induce apoptosis.

Keywords: DNA damage; HSCs; liver fibrosis; sea buckthorn.

MeSH terms

  • Actins / metabolism
  • Animals
  • Apoptosis / drug effects
  • DNA Damage / drug effects
  • Fruit / chemistry
  • Hepatic Stellate Cells / cytology
  • Hepatic Stellate Cells / drug effects*
  • Hepatic Stellate Cells / metabolism
  • Hippophae / chemistry*
  • Humans
  • Liver / drug effects
  • Liver / metabolism
  • Liver Cirrhosis / drug therapy*
  • Liver Cirrhosis / metabolism
  • Liver Cirrhosis / physiopathology
  • Male
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction / drug effects
  • Transforming Growth Factor beta / metabolism

Substances

  • Actins
  • Plant Extracts
  • Transforming Growth Factor beta