Abstract
Here, a novel method was developed for suppressing 129Xe signals in cucurbit[6]uril (CB6) until the trigger is activated by a specific enzyme. Due to its noncovalent interactions with amino-groups and CB6, putrescine dihydrochloride (Put) was chosen for blocking interactions between 129Xe and CB6. Upon adding diamine oxidase (DAO), Put was released from CB6 and a 129Xe@CB6 Hyper-CEST signal emerged. This proposed 129Xe biosensor was then tested in small intestinal villus epithelial cells.
MeSH terms
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Amine Oxidase (Copper-Containing) / metabolism*
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Biosensing Techniques*
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Bridged-Ring Compounds / chemistry
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Bridged-Ring Compounds / metabolism
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Epithelial Cells / enzymology*
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Epithelial Cells / metabolism
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Humans
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Imidazoles / chemistry
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Imidazoles / metabolism
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Intestine, Small / cytology
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Intestine, Small / metabolism
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Intestine, Small / ultrastructure
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Magnetic Resonance Spectroscopy
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Microvilli / enzymology
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Microvilli / metabolism
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Xenon Isotopes
Substances
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Bridged-Ring Compounds
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Imidazoles
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Xenon Isotopes
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Xenon-129
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cucurbit(6)uril
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Amine Oxidase (Copper-Containing)