Isolation and Identification of Potent Antidiabetic Compounds from Antrodia cinnamomea-An Edible Taiwanese Mushroom
- PMID: 30400247
- PMCID: PMC6278467
- DOI: 10.3390/molecules23112864
Isolation and Identification of Potent Antidiabetic Compounds from Antrodia cinnamomea-An Edible Taiwanese Mushroom
Abstract
Antrodia cinnamomea (AC), an edible Taiwanese mushroom, has been recognized as a valuable natural resource with vast biological and medicinal benefits. Recently, the hypoglycemic and anti-diabetic effects of AC were mentioned in several studies. However, no studies have investigated α-glucosidase inhibitors from AC fruiting bodies (ACFB) as they relate to type 2 diabetes (T2D) treatment. The purpose of this study was to gain evidence of potent α-glucosidase inhibitory effects, as well as isolate, identify and characterize the active compounds of ACFB. The MeOH extract of ACFB demonstrated potent α-glucosidase inhibitory activity, and possessed high pH stability (pH 2⁻11) and thermostable properties at 40⁻50 °C. Further purification led to the isolation of eight constituents from ACFB, identified as: 25S-antcin K (1), 25R-antcin K (2), dehydrosulphurenic acid (3), 25S-antcin I (4), 25S-antcin B (5), 25R-antcin B (6), dehydroeburicoic acid (7) and eburicoic acid (8). Notably, the ACFB extract and its identified compounds, except 1, 4, and 6 demonstrated a greater effect (EC50 = 0.025⁻0.21 mg/mL) than acarbose (EC50 = 0.278 mg/mL). As such, these active compounds were determined to be new potent mushroom α-glucosidase inhibitors. These active compounds were also identified on the HPLC fingerprints of ACFB.
Keywords: Antrodia cinnamomea; antcin K; antidiabetic; dehydroeburicoic acid; dehydrosulphurenic acid; eburicoic acid; edible mushroom; α-glucosidase inhibitor.
Conflict of interest statement
The authors declare no conflict of interest.
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References
-
- DeMelo E.B., Gomes A., Carvalha I. α-and β-Glucosidase inhibitors: Chemical structure and biological activity. J. Tetrahedr. 2006;62:10277–10302.
-
- Nguyen V.B., Nguyen Q.V., Nguyen A.D., Wang S.L. Screening and evaluation of α-glucosidase inhibitors from indigenous medicinal plants in Dak Lak Province, Vietnam. Res. Chem. Intermed. 2017;43:3599–3612. doi: 10.1007/s11164-016-2434-x. - DOI
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