Heterologous Expression of the Nybomycin Gene Cluster from the Marine Strain Streptomyces albus subsp. chlorinus NRRL B-24108

Mar Drugs. 2018 Nov 4;16(11):435. doi: 10.3390/md16110435.

Abstract

Streptomycetes represent an important reservoir of active secondary metabolites with potential applications in the pharmaceutical industry. The gene clusters responsible for their production are often cryptic under laboratory growth conditions. Characterization of these clusters is therefore essential for the discovery of new microbial pharmaceutical drugs. Here, we report the identification of the previously uncharacterized nybomycin gene cluster from the marine actinomycete Streptomyces albus subsp. chlorinus through its heterologous expression. Nybomycin has previously been reported to act against quinolone-resistant Staphylococcus aureus strains harboring a mutated gyrA gene but not against those with intact gyrA. The nybomycin-resistant mutants generated from quinolone-resistant mutants have been reported to be caused by a back-mutation in the gyrA gene that restores susceptibility to quinolones. On the basis of gene function assignment from bioinformatics analysis, we suggest a model for nybomycin biosynthesis.

Keywords: heterologous expression; nybomycin biosynthesis; nybomycin gene cluster; secondary metabolites; streptomycetes.

MeSH terms

  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / metabolism*
  • Anti-Bacterial Agents / pharmacology
  • Aquatic Organisms / genetics*
  • Aquatic Organisms / metabolism
  • Computational Biology
  • Genes, Bacterial
  • Methicillin-Resistant Staphylococcus aureus / drug effects
  • Multigene Family*
  • Quinolones / isolation & purification
  • Quinolones / metabolism
  • Quinolones / pharmacology
  • Streptomyces / genetics*
  • Streptomyces / metabolism

Substances

  • Anti-Bacterial Agents
  • Quinolones
  • nybomycin