Prostaglandin E2 directly protects isolated rat gastric surface cell membranes against bile salts

Biochim Biophys Acta. 1987 Aug 20;902(2):217-22. doi: 10.1016/0005-2736(87)90298-7.


Rat gastric surface cell membranes were prepared and the effect of taurocholic acid assessed by ESR spectroscopy using the 16-doxylstearic acid spin label. Taurocholic acid increased the polar part of the spectra, indicating an augmented amount of spin label molecules with a polar environment. Concomitantly, mobility of the spin label molecule was augmented. The effect of taurocholic acid was completely prevented by the previous addition of prostaglandin E2. This suggests a direct protective efficiency of prostaglandin E2 on rat gastric surface cell membranes without the metabolic participation of intact cells.

MeSH terms

  • Animals
  • Bile Acids and Salts / toxicity*
  • Cell Membrane / drug effects
  • Dinoprostone
  • Electron Spin Resonance Spectroscopy
  • Female
  • Gastric Mucosa / drug effects*
  • In Vitro Techniques
  • Prostaglandins E / pharmacology*
  • Rats
  • Rats, Inbred Strains


  • Bile Acids and Salts
  • Prostaglandins E
  • Dinoprostone