Abstract
We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.
MeSH terms
-
Bacterial Outer Membrane Proteins / chemistry*
-
Bacterial Proteins / chemistry*
-
Circular Dichroism / methods*
-
Cytochromes / chemistry*
-
Electron Transport
-
Heme / chemistry*
-
Kinetics
-
Oxidation-Reduction
-
Protein Conformation
-
Shewanella / enzymology*
-
Spectrum Analysis / methods*
Substances
-
Bacterial Outer Membrane Proteins
-
Bacterial Proteins
-
Cytochromes
-
MtrB protein, Shewanella putrefaciens
-
Heme