Identification and localization of glucagon-like peptide-1 and its receptor in rat brain

Endocrinology. 1987 Sep;121(3):1076-82. doi: 10.1210/endo-121-3-1076.

Abstract

The existence and distribution of glucagon-like peptide-1 (GLP-1) and its receptor in rat brain in relation to that of glucagon were examined. The concentration of GLP-1 immunoreactivity (GLP-1-IR), measured by a specific and sensitive RIA established in this study with anti GLP-1 serum (LMT-01), was found to be highest in the thalamus-hypothalamus, followed by the medulla oblongata. The distribution of glucagon-like immunoreactivity was similar to that of GLP-1-IR. However, appreciable glucagon immunoreactivity was detected only in the thalamus-hypothalamus. Gel filtration analysis showed the presence of GLP-1-IR of various molecular weights in the extract of thalamus-hypothalamus including that eluted at the same position as synthetic GLP-1 (1-37); moreover, HPLC analysis also confirmed the presence of GLP-1-IR, eluted at the exact position as synthetic GLP-1 (1-37). The distribution of receptors for GLP-1 corresponded with that of GLP-1-IR in the rat brain, except in the pituitary gland. The distribution of these receptors was also similar to that of glucagon receptors. The thalamus-hypothalamus, pituitary gland, and medulla oblongata were rich in GLP-1 and glucagon-binding sites. The binding affinities of GLP-1 and glucagon were in the nanomolar range [disocciation constant Kd approximately equal to 4 nM]. The presence of specific, high affinity receptors for GLP-1 was confirmed by demonstrating that GLP-1 stimulated cAMP formation in the thalamus-hypothalamus and the pituitary gland. The concentration of GLP-1 required for half-maximal stimulation of cAMP formation in these regions was about 1 nM. These results suggest that GLP-1 may be synthesized in certain parts of the brain and play a role as a neurosignal transmitter.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry*
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Cyclic AMP / biosynthesis
  • Glucagon / analysis
  • Glucagon / metabolism
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptide-1 Receptor
  • Hypothalamus / analysis
  • Male
  • Medulla Oblongata / analysis
  • Peptides / analysis*
  • Peptides / metabolism
  • Pituitary Gland / analysis
  • Rats
  • Rats, Inbred Strains
  • Receptors, Cell Surface / analysis
  • Receptors, Cell Surface / metabolism
  • Receptors, Gastrointestinal Hormone / analysis
  • Receptors, Gastrointestinal Hormone / metabolism
  • Receptors, Glucagon
  • Thalamus / analysis
  • Tissue Distribution

Substances

  • Glp1r protein, rat
  • Glucagon-Like Peptide-1 Receptor
  • Peptides
  • Receptors, Cell Surface
  • Receptors, Gastrointestinal Hormone
  • Receptors, Glucagon
  • Glucagon-Like Peptide 1
  • Glucagon
  • Cyclic AMP