Acrylamide induces adipocyte differentiation and obesity in mice

Chem Biol Interact. 2019 Jan 25;298:24-34. doi: 10.1016/j.cbi.2018.10.021. Epub 2018 Oct 26.


Obesity is a critical risk factor for various diseases including type II diabetes, cerebral infarction, cardiovascular diseases, and various cancers. Acrylamide (ACR) is present in wide range of foods, including fried potato products, root vegetables, bakery products, chips, cakes, cereals, and coffee. In this study, ACR treatment dramatically increased the accumulation of lipid droplets. We also examined expression levels of peroxisome proliferator-activated receptors γ (PPARγ), CCAAT enhancer binding protein α (c/EBPα), and CCAAT enhancer binding protein β (c/EBPβ) as adipogenic transcription factors for adipocyte differentiation. They were dose-dependently increased by treatment of ACR. Moreover, effects of ACR on mitogen-activated protein kinases (MAPKs) and 5' AMP-activated protein kinase (AMPK)-Acetyl-CoA carboxylase (ACC) activation were investigated. Results also showed that ACR induced phosphorylation of MAPKs and AMPK-ACC. ACR also induced expression of adipocyte fatty acid-binding protein (aP2), lipoprotein lipase (LPL), sterol regulatory element-binding protein (SREBP)-1c, and fatty acid synthase (FAS). Exposure of ACR to high fat diet (HFD)-fed mice significantly increased body weight, organ weight, and fat mass of mice. Collectively, these result showed that ACR can act as an enhancer of adipocyte. Therefore, we suggest that up-regulation of the adipogenesis by ACR may be related to the regulation of the MAPKs and AMPK-ACC pathway.

Keywords: Acrylamide; Adipocyte; Adipogenesis; Obesity; Obesogen.

MeSH terms

  • 3T3-L1 Cells
  • AMP-Activated Protein Kinases / metabolism
  • Acrylamide / toxicity*
  • Adipocytes / drug effects*
  • Adipogenesis / drug effects*
  • Adipogenesis / genetics
  • Animals
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Diet, High-Fat / adverse effects
  • Gene Expression Regulation / drug effects
  • Lipid Metabolism / drug effects
  • Lipid Metabolism / genetics
  • MAP Kinase Signaling System / drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity / chemically induced*
  • Obesity / etiology
  • Signal Transduction / drug effects


  • Acrylamide
  • AMP-Activated Protein Kinases