The expression of hybrid HIV:Ty virus-like particles in yeast

Nature. 1987 Sep 3-9;329(6134):68-70. doi: 10.1038/329068a0.

Abstract

The yeast retrotransposon, Ty, encodes a set of proteins that are assembled into virus-like particles, Ty-VLPs (refs 1, 2). These proteins include Ty-VLP structural proteins, a protease that mediates cleavage of primary translation products and a reverse transcriptase. The major structural components of Ty-VLPs are proteolytic products of the primary translation product, p1 (ref. 3). We have recently shown that protein p1 alone can form Ty-VLPs (ref. 3). Here we demonstrate that p1 fusion proteins, comprising most of p1 and part of human immunodeficiency virus (HIV) protein gp120, form hybrid HIV:Ty-VLPs. These hybrid particles provide a rapid means of preparing and evaluating HIV antigens for a variety of immunological purposes.

MeSH terms

  • Base Sequence
  • DNA Transposable Elements*
  • Genes
  • Genes, Fungal*
  • Genes, Viral*
  • HIV / genetics*
  • Nucleic Acid Hybridization
  • Protein Biosynthesis
  • Saccharomyces cerevisiae / genetics*
  • Viral Envelope Proteins / genetics

Substances

  • DNA Transposable Elements
  • Viral Envelope Proteins