Influence of various intensities of 528 Hz sound-wave in production of testosterone in rat's brain and analysis of behavioral changes

Genes Genomics. 2019 Feb;41(2):201-211. doi: 10.1007/s13258-018-0753-6. Epub 2018 Nov 9.


Testosterone is a nuclear androgen receptor ligand that controls multiple pathways in brain. In addition to the active biosynthesis of steroids in classic steroidogenic organs such as gonads, adrenals and placenta, testosterone also produced in astrocyte cells of brain. Testosterone and its level must be regulated in brain; because, it directly and indirectly affects memory and several key behavioral characteristics. The significance of sound waves on key enzymes that regulate levels of testosterone in brain has not been investigated. The aim of our study was to examine physical stress of such as sound on induction behavioral changes in animal models. According to the current study, sound waves with 528 Hz frequency in 100 dB intensity induce testosterone production in brain by enhancing StAR and SF-1 and reducing P450 aromatase gene expression. Frequency of 528 Hz also reduces total concentration of reactive oxidative species in brain tissue. Prolonged exposure to this sound wave showed reduction of anxiety related behaviors in rats. The results reveal that reduced anxiety is related to increased concentration of testosterone in brain. This study may lead to ascertain a possible therapy in which sounds may be utilized to reduce anxiety in individual.

Keywords: 528 Hz frequency; Astrocyte; P450 aromatase; SF-1; StAR; Testosterone.

MeSH terms

  • Animals
  • Aromatase / genetics
  • Aromatase / metabolism
  • Astrocytes / drug effects
  • Astrocytes / metabolism
  • Brain / cytology
  • Brain / metabolism
  • Brain / radiation effects*
  • Cells, Cultured
  • Male
  • Maze Learning
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • RNA Splicing Factors / genetics
  • RNA Splicing Factors / metabolism
  • Rats
  • Reactive Oxygen Species / metabolism
  • Testosterone / metabolism*
  • Ultrasonic Waves*


  • Phosphoproteins
  • RNA Splicing Factors
  • Reactive Oxygen Species
  • Sf1 protein, rat
  • steroidogenic acute regulatory protein
  • Testosterone
  • Aromatase